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Inhibiting the Effects of Fetuin-B Upregulation Using TAK-242

Booth Id:

Cellular and Molecular Biology


Finalist Names:
Sheinin, Eden (School: Yorktown High School)

Low birth weight (LBW) offspring have a high risk of developing cardiovascular and renal diseases such as chronic kidney disease and hypertension, which have no preventative measures or cures. Previous research showed that the protein fetuin-B increases pro-inflammatory cytokine concentration, preventing normal nephrogenesis. Research has also shown that the protein TLR-4 promotes pro-inflammatory cytokine concentration, similarly to fetuin-B, and that TAK-242 is a potential TLR-4 inhibitor. The goal of this study was to determine if treatment with fetuin-B causes glomerular degradation and if treatment with both fetuin-B and TAK-242 prevents such degradation. The hypotheses were that treatment with fetuin-B would cause a significant decrease in glomeruli count and treatment with fetuin-B and TAK-242 would not. To test the hypotheses, the glomeruli of kidneys treated with either fetuin-B or fetuin-B and TAK-242 counted. Fetuin-B treatment caused a -59% change in glomeruli count and treatment with both fetuin-B and TAK-242 caused only a -15% change. The hypotheses were supported: the fetuin-B group experienced a significant attenuation in glomeruli count in comparison to controls (p<.0005), while the TAK-242 + fetuin-B group did not (p>0.05). In the future, TAK-242 treatment during pregnancy could be used to prevent LBW offspring from developing cardiovascular and renal diseases.

Awards Won:
U.S. Agency for International Development: USAID Science for Development First Place Award of $5,000.