Booth Id:
CELL027T
Category:
Cellular and Molecular Biology
Year:
2025
Finalist Names:
Scanlon, Jacob (School: Broadneck High School)
Schneider, Anna (School: Broadneck High School)
Abstract:
Our project determined that resazurin, a synthetic fluorescing agent originally used as a bioburden detector, is a practical fluorescing agent for quantifying NADH in cell-free reactions. We were able to develop a standard curve with a remarkable linear relation between NADH concentration and resazurin fluorescence using NADH solid dissolved into a medium imitating a cell-free environment at incremental concentrations. We then used the curve to reliably quantify NADH in samples of cell-free systems. Our research shows resazurin can be used to further optimize protein production by quantifying NADH in cell-free systems, allowing further scale-up of cell-free biomanufacturing.
Awards Won: