Booth Id:
MCRO025
Category:
Microbiology
Year:
2026
Finalist Names:
Garcia, Isabel (School: Isaac Bear Early College High School)
Abstract:
An estimated $6 billion USD will be lost in 2026 globally due to disease outbreaks in aquaculture facilities, particularly from Vibrio coralliilyticus, a pathogen that infects corals, fish and bivalves. This study explores a novel probiotic-bacteriophage therapy to inhibit V. coralliilyticus strain OCN008 for both prophylactic and post-infection use.
Coral bacterial isolates (P94, B95, R96, Y97 & V44) were combined with bacteriophage 28A in a series of 5 experimental conditions. In initial screenings, combination therapy efficacy was compared to individual therapy efficacy to determine if the combination produced a synergistic effect. It was found that 3 combination therapies (B95+28A, Y97+28A & V44+28A) produced complete bacterial inhibition in 96-well plate assays (p<0.0001) and were more effective than the individual therapies. The combination therapies retained efficacy from 0.1 to 1000x pathogen-treatment ratios, with R96+28A producing complete inhibition (p=0.0043).
Timing of introduction experimentation identified that the combination therapies prove most effective post-infection, followed by prophylactic and simultaneous introduction, respectively, with 3 combination therapies (P94+28A, B95+28A & R96+28A) significantly inhibiting bacterial growth (p<0.0001). Prophylactic in vitro assays identified two combination therapies (Y97+28A & V44+28A) as retaining prophylactic efficacy for up to 7 days (p<0.0001).
In vivo experimentation using Eastern oyster larvae identified P94+28A & Y97+28A as producing significant survival rates (p<0.0001), effectively overcoming the approximate 81% mortality rate observed within 24 hours of infection. Future work is focused on biofilm disruption and microscopy to determine treatment-pathogen interactions within hosts.
Awards Won:
Second Award of $2,400